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KMID : 0606920060140030137
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Biomolecules & Therapeutics
2006 Volume.14 No. 3 p.137 ~ p.142
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Involvement of K+-Cl--Cotransport in the Apigenin-Induced Generation of Reactive Oxygen Species in IMR-32 Human Neuroblastoma Cells
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Kim Min-Ho
Jeong Choon-Sik
Yoon Hye-Ran
Kim Gun-Hee
Lee Yong-Soo
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Abstract
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Apigenin, a natural flavonoid found in a variety of vegetables and fruits, has been shown to possess many biological functions. In this study we investigated the role of apigenin in the production of reactive oxygen species (ROS) through the modulation of activity of K+-Cl--Cotransport (KCC) in IMR-32 human neuroblastoma cells. Apigenin induced Cl--dependent K+ efflux, a hallmark of KCC activity, which was markedly prevented by different kinds of KCC inhibitors (calyculin-A, genistein and BaCl2). These results indicate that KCC is functionally present, and activated by apigenin in the IMR-32 cells. Treatment with apigenin also induced a sustained increase in the level of intracellular ROS. The KCC inhibitors also significantly inhibited the apigenin-induced ROS generation. Taken together, these results suggest that apigenin can modulate ROS generation through the activation of a membrane ion transporter, KCC. These results further suggest that the alteration of KCC activity may play a role in the mechanism of degenerative diseases and/or carcinogenesis in neuronal tissues through the regulation of ROS production.
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KEYWORD
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K+-Cl--cotransport, Reactive oxygen species, Apigenin, IMR-32 cells
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